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Bone marrow mononuclear cells versus mesenchymal stem cells from adipose tissue on bone healing in an Old World primate: can this be extrapolated to humans? Arq. Bras. Med. Vet. Zootec.
Branco,E.; Miranda,C.M.F.C.; Lima,A.R.; Silva,K.S.M.; Cabral,R.M.; Miranda,M.S.; Ohashi,O.M.; Oliveira,E.H.C.; Silva,L.S.C.; Freitas,D.M.M.; Miglino,M.A..
ABSTRACT In veterinary medicine, the cell therapy is still unexplored and there are many unanswered questions that researchers tend to extrapolate to humans in an attempt to treat certain injuries. Investigating this subject in nonhuman primates turns out to be an unparalleled opportunity to better understand the dynamics of stem cells against some diseases. Thus, we aimed to compare the efficiency of bone marrow mononuclear cells (BMMCs) and mesenchymal stem cells (MSCs) from adipose tissue of Chlorocebus aethiops in induced bone injury. Ten animals were used, male adults subjected, to bone injury the iliac crests. The MSCs were isolated by and cultured. In an autologous manner, the BMMCs were infused in the right iliac crest, and MSCs from adipose tissue...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell therapy; Chlorocebus aethiops; Bone injury; Mesenchymal stem cells from adipose tissue.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300917
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Decellularization of placentas: establishing a protocol BJMBR
Leonel,L.C.P.C.; Miranda,C.M.F.C.; Coelho,T.M.; Ferreira,G.A.S.; Caãada,R.R.; Miglino,M.A.; Lobo,S.E..
Biological biomaterials for tissue engineering purposes can be produced through tissue and/or organ decellularization. The remaining extracellular matrix (ECM) must be acellular and preserve its proteins and physical features. Placentas are organs of great interest because they are discarded after birth and present large amounts of ECM. Protocols for decellularization are tissue-specific and have not been established for canine placentas yet. This study aimed at analyzing a favorable method for decellularization of maternal and fetal portions of canine placentas. Canine placentas were subjected to ten preliminary tests to analyze the efficacy of parameters such as the type of detergents, freezing temperatures and perfusion. Two protocols were chosen for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular matrix; Placenta; Decellularization; Acellular biomaterial.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100601
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